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Application  --------------------------------------------------------------------------------------------------------------------The surface charge of nanoparticles gives an indication of their colloidal stability. Nanoparticles having high positive and negative zeta potential show dispersion stability and as a result do not agglomerate on storage. Charge also determines the distribution of these particles in the body and is an important parameter affecting internalization of nanoparticles in their target cells. In one study, it has been reported that uncoated and pullulan-coated SPIONs are