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Detection of species: HumanDetection medium:Serum&Plasma& other biological fluids.
The kit assay Human Cyclin-D1 level in the sample,use Purified Human Cyclin-D1 to coat microtiter plate wells, make solid-phase antibody, then add Cyclin-D1 to wells, Combined Cyclin-D1 antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Cyclin-D1in the samples is then determined by comparing the O.D. of the samples to the standard curve.
