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PurposeThis kit allows for the determination of MMP-9 concentrations in human serum, blood plasma, and other biological fluids.
Principle of the assay
The kit assay human MMP-9 level in the sample,use Purified human MMP-9 antibody to coat microtiter plate wells, make solid-phase antibody, then add MMP-9 wells, Combined MMP-9 which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MMP-9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
