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Principle of testThe kit assay Bovine Col ?level in the sample,use Purified Bovine Col ?antibody to coat microtiter plate wells, make solid-phase antibody, then add Col ? to wells, Combined Col ?antibody which With HRP labeled ,become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Col ? in the samples is then determined by comparing the O.D. of the samples to the standard curve.
