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V8 Protease

FULL NAME
Endoproteinase Glu-C,from Staphylococcus aureus strain V8

ALIAS NAME
V8 Protease

CAS NUMBER
66676-43-5

EC NUMBER
3.4.21.19

DEscriptION
Staphylococcus aureus Protease V8 (Endoproteinase GluC) is a serine proteinase that selectively cleaves peptide bonds C-terminal to glutamic acid residues. It also cleaves at aspartic acid residues.The Optimum pH is 8.0 ~ 8.5. Its enzyme inhibitors are Phosphoric acid diisopropyl ester fluoride (DFP) and alpha 2 - macroglobulin and Na-p-tosyl-L-lysine chloromethyl ketone (TLCK).

SPECIFIC ACTIITY
approx. 20 U/mg at 25°C with Z-Phe-Leu-Glu-4-nitranilide as the substrate (approx. 500 U/mg at 37°C with casein as the substrate).

CONTENT
50 µg/vial,1U/vial

STORAGE
-20°C.

APPLICATION
Protein structure and sequence analysis.