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1 kb ladder plus is ideal for determining the size of double-stranded DNA from 100 to 10,000 base pairs. The ladder consists of 15 linear double-stranded fragments .The 500bp and 3,000bp fragment are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 ul loading, all fragments except 500bp and 3,000bp are 40ng. The 500bp and 3,000bp fragment are 100ng. This ladder is pre-mixed with loading dye and is ready to use.