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PCR and DNA Fragment Purification Kit is designed for rapid and efficient purification of DNA from PCR and other enzymatic reaction mixtures. The kit utilizes a proprietary silicabased membrane
technology in the form of a convenient spin column, eliminating the need for tedious resin
manipulations or toxic phenolchloroform extractions. The PCR and DNA Fragment Purification
Kit effectively removes primers, dNTPs, unincorporated labeled nucleotides, enzymes and salts
from PCR and other reaction mixtures. The kit can be used for purification of DNA fragments
from 50 bp to 40 kb with recovery rates up to 100%. Each purification column has a total binding
capacity of up to 30µg of DNA and the entire procedure takes just 15 min. The purified DNA can
be used in common downstream applications such as sequencing, restriction digestion, labeling,
ligation, cloning, in vitro transcription, blotting or in situ hybridization.
