99% CAS NO 150-25-4 Biological Buffers BICINE N,N-Bis 2-Hydroxyethyl Glycine
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99% CAS NO 150-25-4 Biological Buffers BICINE  N,N-Bis 2-Hydroxyethyl Glycine  

 

Quick Detail:

 

Name

BICINE

Appearance

White crystalline powder

Assay,%

99.99

Solubility(0.1 M aq.)

Clear, colorless solution

Loss on drying,%

0.18

pH(1% aq.)

4.89

Chloride ion,%

0.08

Ultraviolet absorbance(0.1 M aq.)

0.024

Shelf life

two years

Ultraviolet absorbance(0.1 M aq.)

0.020

 

Description:

> Formula: C6H13NO4

> Weight: 163.1717

> MP/BP:190-192(MP)

> Storage: room temperature

> HS:2922499990

> Safety Description: S24/25

 

 

Applications:Biological buffer

 

Synonyms:

DI(HYDROXYETHYL)GLYCINE;DIETHYLOLGLYCINE;DIETHANOLGLYCINE;[BIS-(2-HYDROXY-ETHYL)-AMINO]-ACETIC ACID;BICINE;BICINE BUFFER;LABOTEST-BB LT00452695;n,n-bis(2-hydroxyethyl)-glycin;Diethylolglycine; N,N-Bis(2-hydroxyethyl)glycine; BICINE = N,N-Bis(2-hydroxyethyl)glycine; Bicine, Molecular Biology Grade N,N-Bis(2-hydroxyethyl)glycine, Molecular Biology Grade; Bicine, ULTROL Grade; BICINE buffer Solution; BICINE, [N,N-Bis(2-hydroxyethyl)glycine]; N,N-Di(2-hydroxyethyl)glycine; N,N-Di(2-hydroxyethyl)-glycine; N,N-dihydroxyethylglycine

 

Related categories:Biochemistry;Good's Buffers;Buffer

 

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Determination of melting point

   Instrument:

   Micro melting point apparatus WRS-2A, Melting point tubes

  Determination of step:

Open switch, stable 20min.

First set the starting temperature than the melting point of the low 10 , heating rate 2  / min.

The sample was grind as fine as possible and load in a clean, dry melting point pipe ,until the sample was compressed into about 1cm high .When the actual temperature reaches the set temperature and stabilized . Inserted into the capillary with the sample, the simultaneous determination of three.

 

The determination of PH

Weigh a certain amount of samples into test tube, add a certain amount of water, shake and dissolve, determination of the pH used have been calibrated pH meter.

 

The determination of UV absorbance

  A certain amount of the sample was weighed to the nearest 0.0001g, into 10ml volumetric flask, after constant volume with pure water, at a specific wavelength by 2cm cuvette was measured by UV spectrophotometer.

 

The determination of Loss on drying

  Weigh a sample of approximately 2g (weighing accuracy 0.0001g),Record the weight of m,The sample was placed in a Petri dish constant 

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