1.  General

Ochratoxin A are secondary metabolites of Aspergillus and Penicillium strains, exist widely in grain and grain products, spices and coffee beans and other products. Ochratoxin A has a strong liver and kidney toxicity, and has teratogenic, mutagenic and carcinogenic effects, which is serious harmful to human health.

Thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) has been a common method of Ochratoxin A detection, but sample preparation and samples analysis of this method is time-consuming and exhausting. The use of Ochratoxin A ELISA kit to analyze Ochratoxin A residue of samples is fast and accurate.

This kit is a new generation of fungal toxins detection products in application of ELISA technology, to minimize operating errors and work intensity.

2.  Principle

The test is based on a direct competitive enzyme immunoassay method. The microtiter wells are coated with Ochratoxin antigen. Free Ochratoxin in sample solutions and the immobilized antigen compete for Ochratoxin enzyme conjugate.

While Ochratoxin antibody and HRP secondary antibody (HRP material) combined, via the TMB substrate show color, sample absorbance values ??of Ochratoxin has a negative correlation with its content, compared with the standard curve and then multiplied by the corresponding the dilution factor, can draw Ochratoxin content in samples.

3.  Application

This test kit can be used to detect Ochratoxin in Corn and corn products, soybeans, oats and other crops and beer samples qualitatively and quantitatively.

4.  Technical specifications

Sensitivity:   0.1 ppb

Detection limit:

Corn, soybean, whole wheat etc. …………………………………………………1ppb

Beer ………………………………………………………………………………..0.1ppb

Recovery rate:  

Corn …………………………………………………………………………………80±10%

Whole wheat ………………………………………………………………………..90±10%

Cross-reaction rate

Ochratoxin A …………………………………………………………………………100%

Ochratoxin B ………………………………………………………………………… 9.3%

Ochratoxin C ………………………………………………………………………… 46%

Coefficient of variation:   <10%