Brand:SAVANT
MOQ:10
Quantity:100
Cancer Antigen 125 (CA125)
The Quantitative Determination of CancerAntigen (125) Concentration in Human Serum by a ChemiluminescenceImmunoassay
Summary and explanation of the test
Cancer Antigen 125 (CA-125) is a glycoprotein that occurs inblood as high molecular weight (Mr>200,000). High concentrationsof this antigen are associated with ovarian cancer and a range ofbenign and malignant diseases. Although the specificity andsensitivity of CA-125 assays are somewhat limited, especially inearly diagnosis of ovarian cancer, the assay has found widespreaduse in the differential diagnosis of adnexal masses, in monitoringdisease progression and response to therapy in ovarian cancer, andin the early detection of recurrence after surgery of chemotherapyfor ovarian cancer. Published literature has shown that elevatedserum CA-125 levels can be observed in patients with seriousendometriosis, clear cell and undifferentiated ovarian carcinoma.The serum CA-125 is elevated in 1% of normal healthy women, 3% ofnormal healthy women with benign ovarian diseases, and 6% ofpatients with non-neoplastic conditions.
The employment of several serum references of known CancerAntigen 125(CA-125) levels permits the construction of a doseresponse curve of activity and concentration. From comparison tothe dose response curve, an unknown specimen’s activity can becorrelated with CA-125 concentration.
Materials provided for 96-well microplate
1. | Microplate | 1 piece | 96 test/piece |
2. | Calibrators | 6 tubes | 0.5 mL/tube |
Concentrate:0,10,20,60,160,400U/mL | |||
3. | Label | 1 bottle | 16 mL/bottle |
4. | Substrate A Substrate B | 1 bottle 1 bottle | 6 mL/bottle 6 mL/bottle |
Before using, the substrates A and B are mixed by equalvolume in practicality requisite amount. | |||
5. | Washing buffer | 1 bottle | 20 mL/bottle |
Before using, being diluted to 600ml with distilledwater | |||
6. | Instruction book | 1 piece | 1 piece/kit |
Keeping in 2~8Degree Celsius, is 12 months.
Specimen collection and preparation
The specimens shall be blood serum in type and the usualprecautions in the collection of venipuncture samples should beobserved. For accurate comparison to established normal values, afasting morning serum sample should be obtained. The blood shouldbe collected in a plain red-top venipuncture tube with or withoutgel barrier. Allow the blood to clot. Centrifuge the specimen toseparate the serum from the cells. Samples may be refrigerated at2~8Degree Celsius for a maximum period of five days. If thespecimen can not be assayed within this time, the sample may bestored at temperatures of -20Degree Celsius for up to 30 days.Avoid repetitive freezing and thawing.
Test procedure
The kit is in room temperature for more than 15mins, then
Unit:μL
Microplate Blankwell Standards well Sampleswell |
Calibrators — 25 — Specimens — — 25 Label — 150 150 |
Swirl the microplate gently for 2mins to mix and cover, incubate120 mins at 37Degree Celsius. After washing for 5 times, the substrates are added. |
SubstrateA 50 50 50 SubstrateB 50 50 50 |
Or we also can mix the substrates in a bottle before using, andthen pipette 100μL to each wells. |
Swirl and detect |
Detect Aftersubstrates being pipetted, suggest detecting in 1-3 mins byluminometer, one second per well. |
The data of concentrate and RLU is fitted in linear by log(X)-log(Y).
Expected values for the CA125 (in U/mL)
Healthy and non-pregnant subjects: less than 34U/mL
Performance characteristics
A: Precision
With assay precision is less than 10%, between assay precisionis less than 15%.
B: Accuracy
Between 90%-110%
C: Sensitivity
Less than 4.0U/ml
Manufacturing enterprise: Beijing SavantBiotechnology Co., Ltd.
ELISA / Cancer Antigen 125(CA125)
