Aspergillus Galactomannan ELISA Kit

AspergillusGalactomannan Detection Kit ( Applied on Microplate Reader )

Quantitative detection for Aspergillus galactomannan antigen inhuman serum.

96 Tests

ELISAMethod

ITEM NO. :GM9601

Overview

The AspergillusGalactomannan Detection Kit is an immunoenzymatic indirectcompetitive microplate assay for the detection of Aspergillusgalactomannan antigen in human serum samples. The assay is used toprovide rapid diagnosis reference for the clinicians in identifyingInvasive Aspergillosis (IA) early in the diseaseprocess.

AssayCharacteristics

Principle ofthe Test

This product is an ELISAindirect competition assay for the detection of Aspergillusgalactomannan antigen using specific antibodies. Put thepre-treated human serum sample and the anti-galactomannanantibodies into the wells of the microplate which have been coatedwith the Aspergillus galactomannan. After the wells are incubatedand washed, the enzyme labeled antibodies are added. Following asecond time incubating and washing the wells, TMB substratesolution is added to form a color reaction. The absorbance isdetermined with a microplate reader at 450nm wavelength. Theabsorbance value has a negative correlation with the galactomannancontent. The galactomannan concentration of the sample serum can becalculated from the standard curve, hence the Aspergillusgalactomannan quantitative detection can beperformed.

ClinicalSignificance

The incidence of invasivefungal infection (IFI) by both primary and opportunisticpathogens,has been steadily increasing among hematologicalmalignancy and AIDS patients and the patients receiving aggressivechemotherapy for cancer, bone marrow and organ transplantation oradvanced critical care. The difficulty in diagnosis of theseinfections are associated with significant mortality and morbidity.Since the traditional methods for the fungal infection detectionsuch as blood culture and histopathological diagnosis are eitherless sensitive or not feasible in the critically ill patients,developemnt of untraditional method for the IFI diagnosis is ofgreat importance. 

Among the new diagnostictechniques is the assay for the serum (1-3)-β-d -glucan (BG)detection. Positive in the BG test has been recognized as a veryimportant microbiological evidence in the clinical diagnosticcriteria for invasive fungal infection, which can achieve earlydiagnosis for IFI and dynamic monitoring among patients with risksof IFI. What is more, BG test results provide basis to the use ofmedicine and pharmacodynamics evaluation in clinicaltreatment.

SamplesCollection and Storage

Collect venous bloodsamples 4ml with sterile non-pyrogenic vacuum tubes (red tops). Theserum is then separated from the blood by centrifuge (3000 rmp/minfor 10-15 minutes). Detect within 2 hours. 

Store the kit at 2-8℃ inthe dark. Do not freeze any kit component. The kit can be kept for1 year before opening. once opened the kit is stable for 4 weeks.Avoid contamination during sample collection, transportation, andpreservation. Serum samples should be stored at 2-8℃or frozen at-20℃ or colder within 24 hours after collection. If the samplecould not be detected in time, it should be stored below -20 ℃ toavoid sample contamination and deterioration. The serum samplesshould avoid repeated freezing and thawing.

Because a fungalinfection is a dynamic process repeat testing, typically 2-3 timesper week, improves sensitivity. 

ResultInterpretation